5IQL
Crystal structure of YEATS2 YEATS bound to H3K27cr peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 75.791, 75.791, 119.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.221 - 2.100 |
| R-factor | 0.2094 |
| Rwork | 0.204 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tmp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.842 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.082 | 0.051 | 0.736 |
| Number of reflections | 10507 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 11.4 | 10 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 0.1M Bis-Tris propane, 4.0 M NH4Ac, pH 7.0 |
