5IQF

Aminoglycoside Phosphotransferase (2'')-Ia (CTD of APH(6')-Ie/APH(2'')-Ia) in complex with GDP and Magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-10
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	90.454, 99.075, 92.350
Unit cell angles	90.00, 105.18, 90.00

Refinement procedure

Resolution	89.130 - 2.350
R-factor	0.18244
Rwork	0.180
R-free	0.22783
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5byl
RMSD bond length	0.013
RMSD bond angle	1.537
Data reduction software	iMOSFLM (0.1.27)
Data scaling software	Aimless (0.3.11)
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.130	2.410
High resolution limit [Å]	2.350	2.350
Rmerge	0.139	1.211
Number of reflections	65601
<I/σ(I)>	10.9	1.7
Completeness [%]	100.0	100
Redundancy	7.2	6.9
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5