5IQC

Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) in complex with GMPPNP, Magnesium, and Gentamicin C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-26
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	89.780, 98.890, 93.860
Unit cell angles	90.00, 105.51, 90.00

Refinement procedure

Resolution	90.440 - 2.300
R-factor	0.1815
Rwork	0.179
R-free	0.22853
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5byl
RMSD bond length	0.012
RMSD bond angle	1.449
Data reduction software	iMOSFLM (0.1.27)
Data scaling software	Aimless (0.3.11)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.440	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.092	0.817
Number of reflections	67678
<I/σ(I)>	10.9	1.6
Completeness [%]	96.2	100
Redundancy	4.3	4.3
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5