5IQB
Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) in complex with GMPPNP, Magnesium, and Kanamycin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.430, 99.220, 93.510 |
| Unit cell angles | 90.00, 105.46, 90.00 |
Refinement procedure
| Resolution | 90.130 - 2.300 |
| R-factor | 0.16893 |
| Rwork | 0.167 |
| R-free | 0.21071 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5byl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.496 |
| Data reduction software | iMOSFLM (0.1.27) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.130 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 1.093 |
| Number of reflections | 69207 | |
| <I/σ(I)> | 9.8 | 1.5 |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5 |
