5IQA
Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) in complex with GMPPNP and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-05 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.170, 100.320, 94.060 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 56.040 - 2.150 |
| R-factor | 0.16882 |
| Rwork | 0.167 |
| R-free | 0.20567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uzr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.562 |
| Data reduction software | MOSFLM (0.1.27) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.040 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.108 | 1.211 |
| Number of reflections | 83016 | |
| <I/σ(I)> | 7.8 | 1.4 |
| Completeness [%] | 94.4 | 100 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5 |
