5IP9

Structure of RNA Polymerase II-TFIIF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.91889
Spacegroup name	C 2 2 21
Unit cell lengths	222.820, 392.730, 283.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 3.900
R-factor	0.1628
Rwork	0.162
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3po2
RMSD bond length	0.010
RMSD bond angle	1.260
Data scaling software	XSCALE
Refinement software	BUSTER-TNT (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.540
High resolution limit [Å]	3.450	15.430	3.450
Rmerge	0.206	0.032	4.011
Number of reflections	112721
<I/σ(I)>	9.19	42.41	0.63
Completeness [%]	99.9	95.9	100
Redundancy	7.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	peg 6000, sodium acetate, ammonium acetate, hepes, tcep