5IP8
Lambda-Ru(TAP)2dppz bound to d(CCGGCTCCGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-12-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 |
Unit cell lengths | 47.450, 47.450, 35.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.730 - 1.760 |
R-factor | 0.18949 |
Rwork | 0.188 |
R-free | 0.21957 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 2.692 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.730 | 1.810 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.024 | 0.728 |
Number of reflections | 7868 | |
<I/σ(I)> | 29.8 | 2.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 6 uL of a solution containing 10% v/v MPD, 0.04M Sodium Cacodylate, 0.012M Spermine, 0.08M KCl, 0.02M BaCl2. 1uL 1mM DNA, 1uL 4mM rac-RuTAP2dppz |