5IP7

Structure of RNA Polymerase II-Tfg1 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.97972
Spacegroup name	C 2 2 21
Unit cell lengths	221.220, 392.730, 282.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 3.520
R-factor	0.1542
Rwork	0.154
R-free	0.17970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3po2
RMSD bond length	0.010
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	BUSTER-TNT (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.610
High resolution limit [Å]	3.520	15.740	3.520
Rmerge	0.113	0.025	1.054
Number of reflections	294213
<I/σ(I)>	15.67	62.75	2
Completeness [%]	100.0	96.6	100
Redundancy	7.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG 6000, sodium acetate, ammonium acetate, hepes, tcep