5IP1
Tomato spotted wilt tospovirus nucleocapsid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97906 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.188, 95.960, 71.840 |
Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
Resolution | 35.193 - 2.703 |
R-factor | 0.2136 |
Rwork | 0.210 |
R-free | 0.25520 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.783 |
Phasing software | SHELXDE |
Refinement software | PHENIX (dev_1426) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.980 | 47.980 | 2.870 |
High resolution limit [Å] | 2.700 | 8.010 | 2.700 |
Rmerge | 0.084 | 0.039 | 0.657 |
Rmeas | 0.097 | 0.046 | 0.763 |
Total number of observations | 157868 | ||
Number of reflections | 41185 | 1668 | 7154 |
<I/σ(I)> | 10.8 | 26.18 | 2.13 |
Completeness [%] | 92.3 | 97.1 | 98.5 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES pH7.5, 25% (w/v) PEG 1000, 0.05M NaCl |