5IP1

Tomato spotted wilt tospovirus nucleocapsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-13
Detector	RAYONIX MX225HE
Wavelength(s)	0.97906
Spacegroup name	P 1 21 1
Unit cell lengths	66.188, 95.960, 71.840
Unit cell angles	90.00, 113.37, 90.00

Refinement procedure

Resolution	35.193 - 2.703
R-factor	0.2136
Rwork	0.210
R-free	0.25520
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.783
Phasing software	SHELXDE
Refinement software	PHENIX (dev_1426)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.980	47.980	2.870
High resolution limit [Å]	2.700	8.010	2.700
Rmerge	0.084	0.039	0.657
Rmeas	0.097	0.046	0.763
Total number of observations	157868
Number of reflections	41185	1668	7154
<I/σ(I)>	10.8	26.18	2.13
Completeness [%]	92.3	97.1	98.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M HEPES pH7.5, 25% (w/v) PEG 1000, 0.05M NaCl