5IOO
Accommodation of massive sequence variation in Nanoarchaeota by the C-type lectin fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 143.998, 143.998, 59.261 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 71.999 - 2.521 |
R-factor | 0.2064 |
Rwork | 0.202 |
R-free | 0.24980 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.266 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.999 | 2.610 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.250 | 1.000 |
Number of reflections | 46142 | |
<I/σ(I)> | 12.5 | |
Completeness [%] | 99.8 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 100 mM HEPES (pH 7.5), 50 mM magnesium chloride, 30% PEG monoethyl ether 550 |