5IOI
X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF HUMAN DOUBLECORTIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-09-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 97.719, 97.719, 377.089 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.140 - 2.400 |
R-factor | 0.1979 |
Rwork | 0.196 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bqq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS (January 30 2009) |
Data scaling software | SADABS (2008/2) |
Phasing software | PHASER (2.1.4) |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.140 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 43037 | |
<I/σ(I)> | 15.6 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 21.1 | 20.5 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | N-DCXDD crystals were either obtained out of 20mM CAPS pH 10.5, 100 mM NaCl, 5 mM TCEP or 20 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT |