5INX
A C69-family cysteine dipeptidase in complex with Met and Ala from Lactobacillus farciminis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.650, 182.415, 98.215 |
| Unit cell angles | 90.00, 112.75, 90.00 |
Refinement procedure
| Resolution | 39.314 - 1.681 |
| R-factor | 0.1595 |
| Rwork | 0.158 |
| R-free | 0.18050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iau |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.314 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.071 | 0.330 |
| Number of reflections | 368718 | |
| <I/σ(I)> | 30.29 | 4.83 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 297 | 0.8 M 1,6-hexanediol, 10 mM CoCl2, 100 mM acetate |
