5INW
Structure of reaction loop cleaved lamprey angiotensinogen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97923 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.400, 119.360, 141.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.260 - 2.700 |
R-factor | 0.20774 |
Rwork | 0.204 |
R-free | 0.27698 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ndd |
RMSD bond length | 0.014 |
RMSD bond angle | 1.734 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.260 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.095 | 1.065 |
Number of reflections | 18674 | |
<I/σ(I)> | 12.3 | 1.7 |
Completeness [%] | 90.9 | 84.6 |
Redundancy | 6.3 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 4.6 | 295 | 25% PEG 4000, 0.1M Na acetate pH4.6, 0.2M ammonium sulfate |