5INR
A C69-family cysteine dipeptidase in complex with Ala-Pro from Lactobacillus farciminis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.398, 182.299, 98.335 |
Unit cell angles | 90.00, 112.84, 90.00 |
Refinement procedure
Resolution | 39.254 - 1.652 |
R-factor | 0.16 |
Rwork | 0.159 |
R-free | 0.18220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iau |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.300 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.058 | 0.388 |
Number of reflections | 321818 | |
<I/σ(I)> | 26.14 | 3.93 |
Completeness [%] | 99.2 | 65.9 |
Redundancy | 6.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 0.8 M 1,6-hexanediol, 10 mM CoCl2, 100 mM acetate buffer |