5INE
Crystal structure of the prefusion glycoprotein of LCMV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 262.330, 262.330, 262.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.500 |
| R-factor | 0.2116 |
| Rwork | 0.211 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GPe structure as determined by SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.030 | 0.760 |
| Number of reflections | 19708 | |
| <I/σ(I)> | 17.68 | 0.99 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 57.69 | 44.6 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 0.1M Hepes pH 7.5, 20% PEG 2k and 0.2M lithium sulfate |
