5IND
Crystal structure of HLA-B5801, a protective HLA allele for HIV-1 infection
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.413, 82.280, 157.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.302 - 2.132 |
R-factor | 0.1885 |
Rwork | 0.186 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a1m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.110 |
Rmerge | 0.079 |
Number of reflections | 50306 |
<I/σ(I)> | 23.99 |
Completeness [%] | 100.0 |
Redundancy | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES pH 6.5, 15% PEG4K, 20% 2-proponal |