5IN1
Crystal Structure of the MRG701 chromodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.786, 59.801, 40.616 |
Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
Resolution | 29.901 - 1.400 |
R-factor | 0.1838 |
Rwork | 0.182 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pli |
RMSD bond length | 0.007 |
RMSD bond angle | 1.049 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.400 |
Number of reflections | 28872 |
<I/σ(I)> | 29.05 |
Completeness [%] | 98.6 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 0.1M Sodium acetate pH 4.5, 25% PEG 4000, 0.2M ammonium sulfate |