5IMT
Toxin receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.730, 166.560, 118.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.211 - 2.700 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3r |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.575 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.211 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 48423 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 99.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 8000, zinc acetate, MES pH 6.0, copper sulfate |
