5IMO
Nanobody targeting mouse Vsig4 in Spacegroup P3221
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.906, 49.906, 183.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.220 - 2.100 |
R-factor | 0.2452 |
Rwork | 0.241 |
R-free | 0.28130 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.844 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.220 |
High resolution limit [Å] | 2.100 |
Number of reflections | 16270 |
<I/σ(I)> | 8.96 |
Completeness [%] | 99.6 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Sodium citrate, 20% PEG3000, pH 5.5 |