5IMM
Nanobody targeting mouse Vsig4 in Spacegroup P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.598, 50.285, 163.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.810 - 1.200 |
R-factor | 0.1566 |
Rwork | 0.156 |
R-free | 0.17550 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.330 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.810 |
High resolution limit [Å] | 1.200 |
Number of reflections | 75159 |
<I/σ(I)> | 8.06 |
Completeness [%] | 97.0 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M Ammonium sulfate, 0.1M BIS-Tris, 25% PEG 3350 pH 5.5 |