5IMK

Nanobody targeting human Vsig4 in Spacegroup C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	126.791, 30.369, 84.297
Unit cell angles	90.00, 126.88, 90.00

Refinement procedure

Resolution	42.011 - 1.227
R-factor	0.172
Rwork	0.172
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.011
High resolution limit [Å]	1.227
Number of reflections	74605
<I/σ(I)>	11.93
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Sodium citrate tribasic dehydrate pH5.6, 20% 2-Propanol, 20% PEG 4000