5IMJ
Crystal structure of a Z-ring associated protein from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 64 |
| Unit cell lengths | 109.490, 109.490, 106.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.430 - 3.100 |
| R-factor | 0.22662 |
| Rwork | 0.223 |
| R-free | 0.28263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oez |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.505 | |
| Number of reflections | 15284 | |
| <I/σ(I)> | 33.4 | |
| Completeness [%] | 99.3 | |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Lithium sulfate, HEPES pH 7.8, PEG400 |
