5IM0
Crystal structure of the RNA recognition motif of mRNA decay regulator AUF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.068, 39.411, 93.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.594 - 1.700 |
R-factor | 0.1733 |
Rwork | 0.170 |
R-free | 0.20400 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.987 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 16559 |
<I/σ(I)> | 42.2 |
Completeness [%] | 99.9 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, HEPES |