5ILU
Autoinhibited ETV4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.866, 50.866, 68.621 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.051 - 1.101 |
| R-factor | 0.1739 |
| Rwork | 0.173 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.823 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 2.370 | 1.100 |
| Rmerge | 0.098 | 0.060 | |
| Rmeas | 0.099 | ||
| Rpim | 0.020 | ||
| Total number of observations | 1577832 | ||
| Number of reflections | 42215 | ||
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 37.4 | 51.6 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 293 | 1000 mM (NH4)2HPO4, 100 mM C2H3NaO2, pH 4.3 |
