5ILT

Crystal structure of bovine Fab A01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	80.490, 72.490, 87.420
Unit cell angles	90.00, 107.31, 90.00

Refinement procedure

Resolution	38.422 - 2.000
R-factor	0.214
Rwork	0.211
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k3d
RMSD bond length	0.003
RMSD bond angle	0.640
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.422	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	1.340
Number of reflections	31935
<I/σ(I)>	11.2	1.8
Completeness [%]	98.2	98.5
Redundancy	6.5	1.8
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	20% PEG 6000 0.1M bicine