5ILC
The X-ray structure of the adduct formed in the reaction between hen egg white lysozyme a compound 2, a platin(II) compound containing a O, S bidentate ligand
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-01 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.407, 77.407, 37.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.740 - 1.750 |
R-factor | 0.161 |
Rwork | 0.158 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j1a |
RMSD bond length | 0.020 |
RMSD bond angle | 1.920 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.740 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.040 | 0.220 |
Number of reflections | 11233 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 93.7 | 70.7 |
Redundancy | 6.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | Crystals of hen egg white lysozyme (HEWL)-Pt compound adducts appeared in solutions consisting of 1.1 M NaCl, 0.1 M sodium acetate pH 4.4. Single crystals suitable for X-ray experiments were grown by the hanging drop vapor-diffusion method using a 1:1 ratio of reservoir solution and protein adducts solution with a protein concentration of about 15 mg x ml. |