5ILB
Crystal structure of protease domain of Deg2 linked with the PDZ domain of Deg9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 |
| Unit cell lengths | 106.093, 106.093, 255.956 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.523 - 1.852 |
| R-factor | 0.1912 |
| Rwork | 0.190 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5il9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.922 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | |
| Rmerge | 0.081 | 0.734 |
| Number of reflections | 91028 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.6 | 1.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Magnesium formate, 0.2 M Sodium thiocyanate, 15% w/v PEG 3350 |
