5IL7
Leucine rich repeat domain of the Chlorobium tepidum Roco protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.223, 98.941, 155.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.140 - 2.300 |
R-factor | 0.21649 |
Rwork | 0.214 |
R-free | 0.26082 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 106v |
RMSD bond length | 0.006 |
RMSD bond angle | 1.124 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.140 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.350 |
Number of reflections | 37250 | |
<I/σ(I)> | 13.77 | 4.42 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Na Acetate, 2.2 M Ammonium sulfate |