5IKL
Crystal structure of P. aeruginosa geranyl-CoA carboxylase (GCC), beta subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.722, 105.722, 550.760 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.195 - 2.400 |
R-factor | 0.2184 |
Rwork | 0.215 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.561 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.084 | 0.050 | 0.432 |
Total number of observations | 322453 | ||
Number of reflections | 71918 | ||
<I/σ(I)> | 11.7 | ||
Completeness [%] | 98.6 | 93.4 | 99.4 |
Redundancy | 4.5 | 4.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl (pH 8.0), 60% (v/v) MPD |