5IKJ
Structure of Clr2 bound to the Clr1 C-terminus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763,0.9792,0.9763,0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.499, 103.499, 135.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.325 - 2.300 |
R-factor | 0.2152 |
Rwork | 0.214 |
R-free | 0.24310 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.523 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.325 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.042 | 0.845 |
Number of reflections | 37719 | |
<I/σ(I)> | 24.9 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.6 | 7.9 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | 0.1 M Tris-bicine 30 mM Sodium Fluoride 30 mM Sodium Bromide 30 mM Sodium Iodide 18% Ethylene glycol 9% PEG 8000 |