5IKF

Crystal structure of the C-terminal domain of the Mit1 nucleosome remodeler in complex with Clr1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00004
Spacegroup name	C 1 2 1
Unit cell lengths	105.680, 52.850, 81.970
Unit cell angles	90.00, 115.63, 90.00

Refinement procedure

Resolution	46.217 - 2.800
R-factor	0.2041
Rwork	0.199
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.449
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	autoSHARP
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.059	0.377
Number of reflections	10218
<I/σ(I)>	21	4.5
Completeness [%]	99.8	98.8
Redundancy	6.6	6.5
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M Sodium bromide, 0.1 M Bis-Tris propane pH 7.5, 20% w/v PEG 3350.