5IKF
Crystal structure of the C-terminal domain of the Mit1 nucleosome remodeler in complex with Clr1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.680, 52.850, 81.970 |
| Unit cell angles | 90.00, 115.63, 90.00 |
Refinement procedure
| Resolution | 46.217 - 2.800 |
| R-factor | 0.2041 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.449 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.059 | 0.377 |
| Number of reflections | 10218 | |
| <I/σ(I)> | 21 | 4.5 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Sodium bromide, 0.1 M Bis-Tris propane pH 7.5, 20% w/v PEG 3350. |
