5IKB
Crystal structure of the kainate receptor GluK4 ligand binding domain in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-07 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.500, 69.870, 162.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.690 - 2.050 |
| R-factor | 0.19494 |
| Rwork | 0.191 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxt |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.805 |
| Data reduction software | xia2 (3.6.3) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.690 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.136 | 0.709 |
| Number of reflections | 16260 | |
| <I/σ(I)> | 8.9 | 2 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 15 % glycerol, 25.5 % PEG4000, 0.17 M ammonium sulfate |
