5IK5
Laminin A2LG45 C-form, G6/7 bound.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.390, 110.740, 123.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.950 - 1.390 |
| R-factor | 0.1504 |
| Rwork | 0.149 |
| R-free | 0.17680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.895 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.950 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.035 | 0.422 |
| Number of reflections | 81183 | |
| <I/σ(I)> | 17.3 | 1.3 |
| Completeness [%] | 83.7 | 35.8 |
| Redundancy | 3.9 | 1.6 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 100mM HEPES/MOPS 120mM Glucuronic Acid 30% PEG8k/Glycerol mix |
