5IK4
Laminin A2LG45 C-form, Apo.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.690, 111.360, 124.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.680 - 1.270 |
| R-factor | 0.1598 |
| Rwork | 0.159 |
| R-free | 0.18190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyk |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.176 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.680 | 1.315 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.051 | 1.893 |
| Number of reflections | 128754 | |
| <I/σ(I)> | 19.3 | 1.3 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 12.9 | 11.8 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 100mM HEPES/MOPS buffer 120mM Glucuronic acid 30% PEG8k/Glycerol mixture |
