5IJX
Crystal Structure of a C-terminally truncated Coccidioides posadasii mitochondrial tyrosyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.173, 75.785, 52.316 |
Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
Resolution | 27.679 - 2.630 |
R-factor | 0.2079 |
Rwork | 0.206 |
R-free | 0.23560 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | autoSHARP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.630 |
Number of reflections | 12810 |
<I/σ(I)> | 40.9 |
Completeness [%] | 94.1 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 10 mM HEPES-NaOH pH 7.0, 8% PEG 3350, 1% isopropanol, 1 mM L-tyrosine, 12% glycerol |