5IJV
Crystal structure of bovine Fab E03
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.244, 221.737, 67.947 |
| Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
| Resolution | 49.100 - 2.200 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3d |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.870 |
| Number of reflections | 89569 | |
| <I/σ(I)> | 12 | 1.9 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 4 | 3.9 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 0.2M NaCl 16.5% PEG 8000 0.1M phosphate-citrate buffer |
