5IJP
Crystal structure of the SPX domain of Chaetomium thermophilum Vtc4 in complex with inositol hexakisphosphate (InsP6).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-02 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 62 |
Unit cell lengths | 74.200, 74.200, 74.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.630 - 2.750 |
R-factor | 0.2429 |
Rwork | 0.243 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iig |
RMSD bond length | 0.007 |
RMSD bond angle | 0.840 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 6115 | |
<I/σ(I)> | 43 | 2.55 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 82.4 | 86 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | 13% PEG 3350, 6% Tacsimate |