5IJL
D-family DNA polymerase - DP2 subunit (catalytic subunit)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9840 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.820, 106.200, 110.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.800 - 2.190 |
R-factor | 0.2008 |
Rwork | 0.199 |
R-free | 0.23460 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.800 |
High resolution limit [Å] | 2.190 |
Number of reflections | 63302 |
<I/σ(I)> | 8.5 |
Completeness [%] | 99.6 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 60mM MES pH 5.6, 300mM NaCl, 6% PEG6000 |