5IJH
Structure of the SPX domain of the human phosphate transporter XPR1 in complex with a sulfate ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.976250 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.950, 80.320, 97.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.280 - 2.430 |
R-factor | 0.2034 |
Rwork | 0.201 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iig |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.430 | 2.430 |
Number of reflections | 20166 | |
<I/σ(I)> | 16.65 | 1.75 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.6 | 7.3 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.4M AmSO4, 0.1M Tris, 16% glycerol |