5IJ3
SrpA adhesin in complex with sialyl T antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.911, 46.766, 65.169 |
| Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
| Resolution | 27.815 - 1.700 |
| R-factor | 0.1867 |
| Rwork | 0.186 |
| R-free | 0.20690 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.900 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.055 | 0.032 | 0.460 |
| Rmeas | 0.060 | ||
| Rpim | 0.022 | ||
| Total number of observations | 400540 | ||
| Number of reflections | 56465 | ||
| <I/σ(I)> | 15.1 | ||
| Completeness [%] | 99.7 | 99.7 | 97 |
| Redundancy | 7.1 | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | calcium acetate, sodium cacodylate, PEG 8000 |
