5IJ1
SrpA adhesin in complex with sialyllactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-12-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.522, 46.769, 64.761 |
Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
Resolution | 42.559 - 1.800 |
R-factor | 0.1767 |
Rwork | 0.176 |
R-free | 0.19820 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.058 | 0.025 | 0.475 |
Rmeas | 0.066 | ||
Rpim | 0.032 | ||
Total number of observations | 188088 | ||
Number of reflections | 45316 | ||
<I/σ(I)> | 14 | ||
Completeness [%] | 94.4 | 98.9 | 64 |
Redundancy | 4.2 | 4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | calcium acetate, sodium cacodylate, PEG 8000 |