5IIN

Crystal structure of the pre-catalytic ternary extension complex of DNA polymerase lambda with an 8-oxo-dG:dC base-pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	56.471, 62.718, 140.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.359 - 2.150
R-factor	0.1893
Rwork	0.187
R-free	0.23140
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.200
High resolution limit [Å]	2.150
Number of reflections	32307
<I/σ(I)>	16.5
Completeness [%]	98.1
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.2 M ammonium acetate, 0.1 M sodium citrate pH 4.8, and 3-5% PEG 4000