5IIN
Crystal structure of the pre-catalytic ternary extension complex of DNA polymerase lambda with an 8-oxo-dG:dC base-pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.471, 62.718, 140.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.359 - 2.150 |
R-factor | 0.1893 |
Rwork | 0.187 |
R-free | 0.23140 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.200 |
High resolution limit [Å] | 2.150 |
Number of reflections | 32307 |
<I/σ(I)> | 16.5 |
Completeness [%] | 98.1 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.2 M ammonium acetate, 0.1 M sodium citrate pH 4.8, and 3-5% PEG 4000 |