5IIG
Structure of the SPX-TTM domain fragment of the yeast inorganic polyphophate polymerase Vtc4 (form A).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 145.390, 145.390, 71.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.990 |
| R-factor | 0.2452 |
| Rwork | 0.244 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3r |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.170 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 16084 | |
| <I/σ(I)> | 20.22 | 1.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 12.2 | 12.2 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 1.5M Li2SO4 |
