5II9
Monoclinic crystal structure of red abalone lysin at 2.11 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.028921 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.190, 69.340, 182.750 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 27.340 - 2.110 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lis |
RMSD bond length | 0.004 |
RMSD bond angle | 0.615 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.339 | 2.190 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.061 | 0.379 |
Number of reflections | 52122 | |
<I/σ(I)> | 10.45 | 2.33 |
Completeness [%] | 92.0 | 89 |
Redundancy | 2.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 5% PEG 4000, 0.1 M sodium citrate, 0.2 M sodium acetate |