5II5
Crystal structure of red abalone VERL repeat 1 at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.090, 107.090, 196.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.837 - 1.800 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3set 3SEX and 4WRN |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.337 |
| Data reduction software | XDS (20141103) |
| Data scaling software | XDS (20141103) |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev-1894_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.837 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 1.538 |
| Number of reflections | 61781 | |
| <I/σ(I)> | 17.51 | 1.08 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.9 | 5.9 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 40% PEG 600, 0.1M CHES |
