5IHZ

Crystal structure of anti-gliadin 1002-1E01 Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	74.270, 147.810, 95.230
Unit cell angles	90.00, 103.50, 90.00

Refinement procedure

Resolution	78.472 - 1.640
R-factor	0.2018
Rwork	0.200
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q9q
RMSD bond length	0.010
RMSD bond angle	1.272
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.600	1.680
High resolution limit [Å]	1.640	1.640
Number of reflections	240238
<I/σ(I)>	18.3
Completeness [%]	98.8
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 %w/v Polyethylene Glycol 3350, 0.200 M Potassium Sulphate