5IHY
The crystal structure of Bacillus subtilis SeMet-YpgQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-24 |
Detector | ADSC Quantum Q270r |
Wavelength(s) | 0.97937 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.487, 53.087, 83.388 |
Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.21734 |
Rwork | 0.215 |
R-free | 0.26145 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 14383 | |
<I/σ(I)> | 29.4 | |
Completeness [%] | 99.1 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 40% PEG 600, 0.1 M sodium citrate |