5IHX

Crystal Structure of a C-terminally truncated Aspergillus nidulans mitochondrial tyrosyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97947
Spacegroup name	P 21 21 21
Unit cell lengths	69.461, 84.950, 164.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.020 - 2.298
R-factor	0.1817
Rwork	0.180
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ts1
RMSD bond length	0.003
RMSD bond angle	0.720
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.0)
Refinement software	PHENIX (dev_1535)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.298	6.240	2.300
Rmerge	0.112	0.044	0.724
Number of reflections	44001
<I/σ(I)>	13
Completeness [%]	100.0	100	100
Redundancy	6.8	6.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	295	10 mM Tris-HCl pH 8.5, 8% PEG 8000, 1 mM L-tyrosine, 12% glycerol