5IGS
Macrolide 2'-phosphotransferase type I - complex with guanosine and oleandomycin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-13 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 45.170, 45.170, 247.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.118 - 1.380 |
R-factor | 0.1553 |
Rwork | 0.155 |
R-free | 0.16650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5igr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.343 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.118 | 1.420 | |
High resolution limit [Å] | 1.380 | 6.170 | 1.380 |
Rmerge | 0.028 | 0.015 | 0.477 |
Number of reflections | 59173 | ||
<I/σ(I)> | 27.51 | 72.74 | 2.15 |
Completeness [%] | 95.5 | 96.9 | 71.7 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 295 | 0.1 M Na citrate, 15% isopropanol, 27% PEG 4000 |