5IGR
Macrolide 2'-phosphotransferase type I - complex with GDP and oleandomycin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-03 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 45.110, 45.110, 246.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.066 - 1.600 |
R-factor | 0.1591 |
Rwork | 0.157 |
R-free | 0.19570 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.662 |
Data reduction software | XDS (March 5, 2012) |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1627) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.066 | 1.640 | |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.040 | 0.022 | 0.432 |
Number of reflections | 39609 | ||
<I/σ(I)> | 27.15 | 62.46 | 3.16 |
Completeness [%] | 99.1 | 97.5 | 93.4 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.8 | 277 | 0.1 M Na citrate, 18% isopropanol, 27% PEG 4000, 0.2 M ammonium acetate |