5IGR

Macrolide 2'-phosphotransferase type I - complex with GDP and oleandomycin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-03
Detector	RAYONIX MX-225
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	45.110, 45.110, 246.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.066 - 1.600
R-factor	0.1591
Rwork	0.157
R-free	0.19570
RMSD bond length	0.016
RMSD bond angle	1.662
Data reduction software	XDS (March 5, 2012)
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (dev_1627)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.066		1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.040	0.022	0.432
Number of reflections	39609
<I/σ(I)>	27.15	62.46	3.16
Completeness [%]	99.1	97.5	93.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.8	277	0.1 M Na citrate, 18% isopropanol, 27% PEG 4000, 0.2 M ammonium acetate